IKKg
N-terminal cMyc and GST-tagged, recombinant, human IKKγ, full length, expressed by baculovirus in Sf21 insect cells.
Biological information
Background
IKKγ, also known as NEMO (NF-kappa B essential modulator), is a regulatory subunit of the IκB kinase (IKK) complex. The IKK complex is responsible for phosphorylating and degrading the inhibitor of NF-κB (IκB) proteins, which allows for the activation of the transcription factor NF-κB.
IKKγ is essential for the function of the IKK complex, and its deficiency results in impaired NF-κB activation and immune dysfunction. IKKγ has also been implicated in other cellular processes, such as the regulation of DNA damage responses and the maintenance of genomic stability.
Dysregulation of IKKγ has been associated with several human diseases, including immunodeficiency syndromes, autoimmune diseases, and cancer. Therefore, IKKγ is a potential therapeutic target for the treatment of these diseases.
Target class
Ubiquitin
Family
Substrate
Accession number
GenBank AF091453
Target Name
IKKg
Target Alias
AMCBX1, EDAID1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, SAIDX, ZC2HC9
Origin
Human
Theori. MW
76 kDa
Affinity tag
N-terminal cMyc and GST-tag
Product specifications
Expression system
expressed in insect cells
Purity
81%
Purification method
Glutathione sepharose
Sample Buffer
0.98 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
IKKγ, also known as NEMO (NF-kappa B essential modulator), is a regulatory subunit of the IκB kinase (IKK) complex. The IKK complex is responsible for phosphorylating and degrading the inhibitor of NF-κB (IκB) proteins, which allows for the activation of the transcription factor NF-κB.
IKKγ is essential for the function of the IKK complex, and its deficiency results in impaired NF-κB activation and immune dysfunction. IKKγ has also been implicated in other cellular processes, such as the regulation of DNA damage responses and the maintenance of genomic stability.
Dysregulation of IKKγ has been associated with several human diseases, including immunodeficiency syndromes, autoimmune diseases, and cancer. Therefore, IKKγ is a potential therapeutic target for the treatment of these diseases.
Compound name
Ubiquitin
Catalog number
23-060
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-060
Datasheet