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COMPANY
Expertise
Mission
Technology
Case Studies
Adenosine Receptor (A2A)
ABC Transporter (BmrA)
Bacteriorhodopsin
Bcl-2 Associated Athanogene 3 (BAG3)
K-Cl co-transporter 2 (KCC2)
Influenza A – H1N1 vaccinal preparation
Influenza Matrix Protein 2 (M2)
Voltage-gated Sodium Channel (Nav1.7)
Track Record
About Us
CALIXAR’s Partners
History
Business Model
Media
Press Releases
Related News
Events
PIPELINE
Undisclosed Ion Channel
Undisclosed Receptor
Adenosine receptor A2A
Melatonin receptor type 1A
Multidrug efflux pump subunit AcrB
Multidrug resistance ABC transporter ATP-binding/permease protein BmrA
Solute carrier family 12 member 5
Vaccine Programs
HIV
Leptospirosis
Influenza
PRODUCTS
Purified Membrane Proteins
GPCRs
Adenosine receptor A2A
Melatonin receptor type 1A
Orphan GPCR
Ion Channels
Solute carrier family 12 member 5
Transporters
Multidrug efflux pump subunit AcrB
Multidrug resistance ABC transporter
ATP-binding/permease protein BmrA
Vaccine Programs
HIV
Leptospirosis
Influenza
Chickungunya
Reagents and Kits
Proprietary Mild Detergents
CALX8
DDG
DDTAC
DLAC
FLAC6
ODG
Proprietary Stabilization Reagents
CALXGLUK
DDLAC
FTAC6
FTAC8
Kits
C2B Crystallization Kit
Solubilization & Stabilization Kit
SERVICES
Production
Expression
Isolation
Identification
Proteomic Studies
Target Identification
Ligand Identification
Understanding mechanism of action for targets or ligands
Structure Determination
Cryo EM studies
Crystallogenesis
Xray studies
3D Structure Determination
Biological Assays
Ligand Binding Assays
Thermal Stability Assays
Structural Assays
Functional Assays
Stabilization Assays
Design your project
APPLICATIONS
Drug Discovery
Deorphanization
Target Identification
Target Validation
High Throughput Screening (HTS)
Structure Based Drug Design (SBDD)
Fragment Based Drug Design (FBDD)
Structural Studies (Cryo EM, Xrays, etc)
Antibody Discovery
Phage Display
Yeast Display
Immunization
Vaccine Discovery
Antigen Discovery
Vaccine Formulation
Anti Bacterial Based Vaccine
Anti Viral Based Vaccine
Cell Based Vaccine
Subunit Antigen Vaccine
Split Virus Based Vaccine
Immunoassays
NEWS
CONTACT
Menu
COMPANY
Expertise
Mission
Technology
Case Studies
Adenosine Receptor (A2A)
ABC Transporter (BmrA)
Bacteriorhodopsin
Bcl-2 Associated Athanogene 3 (BAG3)
K-Cl co-transporter 2 (KCC2)
Influenza A – H1N1 vaccinal preparation
Influenza Matrix Protein 2 (M2)
Voltage-gated Sodium Channel (Nav1.7)
Track Record
About Us
CALIXAR’s Partners
History
Business Model
Media
Press Releases
Related News
Events
PIPELINE
Undisclosed Ion Channel
Undisclosed Receptor
Adenosine receptor A2A
Melatonin receptor type 1A
Multidrug efflux pump subunit AcrB
Multidrug resistance ABC transporter ATP-binding/permease protein BmrA
Solute carrier family 12 member 5
Vaccine Programs
HIV
Leptospirosis
Influenza
PRODUCTS
Purified Membrane Proteins
GPCRs
Adenosine receptor A2A
Melatonin receptor type 1A
Orphan GPCR
Ion Channels
Solute carrier family 12 member 5
Transporters
Multidrug efflux pump subunit AcrB
Multidrug resistance ABC transporter
ATP-binding/permease protein BmrA
Vaccine Programs
HIV
Leptospirosis
Influenza
Chickungunya
Reagents and Kits
Proprietary Mild Detergents
CALX8
DDG
DDTAC
DLAC
FLAC6
ODG
Proprietary Stabilization Reagents
CALXGLUK
DDLAC
FTAC6
FTAC8
Kits
C2B Crystallization Kit
Solubilization & Stabilization Kit
SERVICES
Production
Expression
Isolation
Identification
Proteomic Studies
Target Identification
Ligand Identification
Understanding mechanism of action for targets or ligands
Structure Determination
Cryo EM studies
Crystallogenesis
Xray studies
3D Structure Determination
Biological Assays
Ligand Binding Assays
Thermal Stability Assays
Structural Assays
Functional Assays
Stabilization Assays
Design your project
APPLICATIONS
Drug Discovery
Deorphanization
Target Identification
Target Validation
High Throughput Screening (HTS)
Structure Based Drug Design (SBDD)
Fragment Based Drug Design (FBDD)
Structural Studies (Cryo EM, Xrays, etc)
Antibody Discovery
Phage Display
Yeast Display
Immunization
Vaccine Discovery
Antigen Discovery
Vaccine Formulation
Anti Bacterial Based Vaccine
Anti Viral Based Vaccine
Cell Based Vaccine
Subunit Antigen Vaccine
Split Virus Based Vaccine
Immunoassays
NEWS
CONTACT
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Design your project
Design your project v2
Your Target / Construct
PROTEIN NAME, UNIPROT ID.
FINAL APPLICATION
SEQUENCE
FULL-LENGTH
ECTODOMAIN
TAGS
Calixar offers multiple
cleavable tags
(His, Flag, Strep, Avi…) and/or
protein-fusion
(GFP, RFP…), and will assist you to select optimal options for your project.
Your Production Process
EXPRESSION SYSTEM
E. COLI
YEAST
INSECT CELLS
MAMMALIAN CELLS (CHO)
HUMAN CELLS (HEK)
PROTEIN SOLUBILIZATION, STABILIZATION & PURIFICATION
Calixar’s purification process includes
solubilization and stabilization screening
in various proprietary and commercial detergents and/or SMALPs.
1- or 2-step affinity purification
followed by a
size-exclusion chromatography
ensures >90% purity degree.
QUALITY CONTROLS
Calixar’s quality control process includes:
✓
Purity evaluation
✓ Protein
quantification
✓
Homogeneity
determination
✓
Cryo-stability assay
In addition, Calixar can develop specific protein characterizations such as:
•
Thermal shift assay
•
Functional assay
(enzymatic activity, proteoliposome ion flux assay
•
Ligand binding assay
(radiobinding, fluorescence polarization, microscale thermophoresis
PRODUCTION SCALE
From
µg- to mg-scale
production, Calixar will fit all your needs of proteins for your R&D developments.
FINAL FORMAT
Based on your final application, Calixar is able to deliver your purified target in various formats such as
micelles of detergent, SMALPs, amphipols, nanodiscs
and
proteoliposomes.
Your Information
FIRST NAME
LAST NAME
EMAIL
COMPANY / INSTITUTION
LOCATION
SUBMIT REQUEST