Case Study:
Voltage-gated Sodium Channel (Nav1.7)

Another highly druggable target is Nav1.7. It is a voltage-gated sodium channel and plays a critical role in nociception and pain. We could produce this target in CHO cells and could see that in Native-PAGE it was not aggregated.

Size exclusion chromatography and negative stain electron microscopy could confirm that our solubilized/ purified Nav1.7 (using proprietary detergent and methodology) was not aggregated.

It is interesting to notice that when we heat the Nav1.7 purified sample, we lose the ligand binding in this ProTx-II saturation binding assay. Functional antibodies were generated from this preparation (undisclosed).

The same type of work, successfully performed  with Nav 1.7, was also done on other highly druggable channels belonging to the Nav or P2X families or others.

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