SOLUBILIZATION AND STABILIZATION SCREENING KIT

CALIXAR™ Solubilization & Stabilization Kit

CALIXAR’s screening kit helps to find the most suitable combination of CALIXAR’s reagents to extract and stabilize membrane proteins. 

CALIXAR™ Solublization & Stabilization Kit

Reach high solubility rate, increase your protein stability for your scientific projects with accurate results to discover new drugs (small molecules, antibodies, …) and to open new therapeutic pathways.

Compound name: CALIXAR™ Screening Kit

Catalogue number: CALIXAR-K1

Including reagents:  5 soft proprietary detergents and 5 soft proprietary stabilization reagents

Chemical structures:  CALX8, DDTAC, DDG, DLAC, FLAC6, FTAC6, FTAC8, DDLAC, CALXCHOL, CALXGLUK

MW: 497.6 – 2582 (g/mol)

Volume: CALIXAR-K1 is presented as a 10 x 100 µL microfuge tubes

For a typical 10-wells experiment: Add 250 µg of membrane protein sample, 10 µL of soft proprietary detergent and complete with buffer of choice to 50 µL.

For stabilization experiment also add 10µL of soft proprietary and stabilization reagent. 

Stability: Long term storage at -20 ° C.

Solubility: Provided in solution.

Working pH: Maintain pH between 6-9 for optimal results.

User guide and certificate of analysis will be including in your package.

Ships in 7 business days

You can purchase each reagent individually from our online catalog.

First in market

CALIXAR is the first to produce native, wild-type, unmutated and untruncated targets.

Better discovery

Develop reliable and effective drugs and antibodies as they are not locked in any specific conformation.

Why choose us?

We use proprietary technology and custom-built method to give you the edge

CALIXAR’s solubilization and stabilization screening kit helps to extract and stabilize membrane therapeutic targets (GPCRs, ion channels, transporters, enzymes, …). This kit includes 5 soft proprietary detergents and 5 soft proprietary stabilization reagents to improve trials and research involving membrane proteins.

10 unique proprietary reagents produced by CHEM2STAB, a joint research lab between CALIXAR and Avignon University.

With 10 tests, our screening assay kit can be used to determine the best reagent or the most suitable combination of reagents to extract, solubilize and stabilize native and functional membrane proteins in a solution.  

Thanks to these reagents, our clients can obtain pure and functional membrane proteins with high medical relevance to discover new drugs (small molecules, antibodies,  vaccines, …). 

CALIXAR’s screening assay kit is produced with 10 unique soft proprietary detergents and soft proprietary stabilization reagents. These molecules are specifically designed to extract and stabilize membrane proteins while preserving their functionalities.

CALIXAR™ Solubilization and Stabilization Screening Kit is used for research, drug discovery, and structural studies that are used in the life science fields. Particularly adapted for biotechnology companies, as well as for academic teams (structural biologists, pharmacologists, biochemists, virologists).

  • Antibodies (including nanobodies, scaffold proteins, aptamers)
  • Small molecules
  • 3D Structures (cryoEM, XRay crystallography, NMR, SANS, SAXS)
  • Drug discovery (Screening: HTS, FBDD, SBDD; Hit and lead validation)
  • Antibody discovery (Immunization and display technologies)
  • Clinical stage (drug validation on reliable native DDLAC)

References

ACS Publications

Lactobionamide Surfactants with Hydrogenated, Perfluorinated or Hemifluorinated Tails

Lebaupain F. et al. 2006

Febs Letters

New tensio-active molecules stabilize a human G protein-coupled receptor in solution.

Damian, M. et al. 2007

ACS Publications

Glucose-Based Surfactants with Hydrogenated, Fluorinated, or Hemifluorinated Tails: Synthesis and Comparative Physical-Chemical Characterization

Abla M et al. 2008

Journal of Bioenergetics and Biomembranes

Hydrogenated and fluorinated surfactants derived from Tris(hydroxymethyl)-acrylamidomethane allow the purification of a highly active yeast F1-F0 ATP-synthase with an enhanced stability.

Talbot J.-C., et al. 2009

Institut de Biologie Structurale

Structure and Stability of Integral Membrane Protein and Phage Assemblies

Breyton C et al. 2013

Proprietary patent

Method for the synthesis of steroidic calixarene compounds and their use for extraction, solubilization and/or stabilization of native and functional membrane proteins.

Dauvergne J. 2014

Journal of Colloid and Interface Science

Micellar and biochemical properties of a propyl-ended fluorinated surfactant designed for membrane-protein study

Abla M et al. 2015

Proprietary patent

Synthesis of amphiphilic calixarene glycoside detergents and use of same for extracting and stabilizing native functional membrane proteins

Dauvergne J. 2015

ANALYTICAL BIOCHEMISTRY

Novel systematic detergent screening method for membrane proteins solubilization

Desuzinges Mandon E. et al. 2017 

LANGMUIR

Hydrogenated Diglucose Detergents for Membrane-Protein Extraction and Stabilization

Pierre Guillet et al. 2019 

CHEMISTRY SELECT

Glycosylated Amphiphilic Calixarene-Based Detergent for Functional Stabilization of Native Membrane Proteins

Julien Dauvergne et al. 2019