Purified Membrane Proteins

CALIXAR patented technological platform allows the isolation in solution of full length and pure to highly pure membrane proteins – GPCRs, Ion Channels, Transporters, Receptors, Viral Proteins, … – while keeping their structural and functional integrity (no refolding step and no mutagenesis).

This innovative approach allowed the native isolation of membrane proteins which can be used for antibody development, vaccine formulation and/or drug discovery (FBDD, SBDD).

 

The following wild type and pure to highly pure purified membrane proteins are commercially available off-the-shelf:

 

A2A: Adenosine Receptor

A class A GPCR involved in regulating myocardial blood flow/hypertension, the regulation of glutamate and dopamine release. A2A is a good therapeutic candidate for insomnia, pain, depression, drug addiction and Parkinson’s disease.  Protein has a N-terminal Step tag II and an 8X His tag followed by a TEV protease cleavage site.

MT1: Melatonine Receptor

Melatonin receptor 1 (MT1) is a class A GPCR that modulates neuronal firing, arterial vasoconstriction, cell proliferation in cancer cells, and reproductive and metabolic functions.  MT1 is a good therapeutic candidate for insomnia, circadian sleep disorders, depression, cardiovascular regulation, cancer and neurodegenerative diseases.  Protein has a strep tag II and 10X His tag at the N-terminus followed by a TEV protease cleavage site.

BmrA: Bacilus subtilis multidrug resistance ABC transporter ATP-binding Protein (BmrA) is an efflux protein and ABC transporter that acts as a mechanical pump for ATP dependent drug efflux. BmrA is a good target for multi-drug resistance studies. The protein has a N-terminal 6X His tag.

AcrB : Acriflavine resistance Protein B

Acriflavine resistance Protein B (AcrB) is a bacterial multidrug exporter. AcrB is a good target for indications of bacterial resistance. Protein has a C-terminal 6X His tag.

Nav1.7: Voltage-gated sodium channel

Voltage-gated sodium channel (Nav1.7) is involved in the generation and conduction of action potentials. Nav1.7 is a good candidate for pain indications. Protein has a C-terminal TEV cleavage site followed by a 10X His tag and a 2X Strep-Tag II.

 

Check out more details on our off-the-shelf membrane proteins in the following table:

Target Name (1,2)
Family Expression System
 Quantity (3)
Price (4)(for 10µg) Datasheet Order (5)
A2A
(Adenosine receptor A2A)
Class A GPCR Sf9 From 10µg up to mg scale 1000 € PDF email
MT1
(Melatonin receptor 1)
Class A GPCR Sf9 1000 € PDF email
BmrA
(Bacilus subtilis multidrug resistance ABC transporter ATP-binding Protein)
ABC Transporter E. coli 200 € PDF
email
AcrB
(Acriflavine resistance Proteine B)
Multidrug Efflux Transporter E. coli 200 € PDF email
Nav1.7
(Voltage-gated sodium channel)
Ion Channel CHO Temporarily unavailable PDF email
KCC2
(Neuron-specific K-Cl Cotransporter)
Ion Channel HEK Coming soon
M2
(M2 influenza Proton channel)
Ion Channel CHO Coming soon

All these proteins are also available under the proteoliposome format
Membrane preparations of each target can be ordered on demand
Specific quantities can be provided upon request depending on the target
Prices do not include customs and shipping costs
5 Minimum order amount must be 1000€

 

Other proteins are currently being produced and optimized in ours labs throught internal research efforts (see our Pipeline) and we also have the capacity to work on any membrane protein targets through our fee-for-service model.

Each proteins listed above are for research use only. If you need to use it for developments including drug / biodrug discovery, antibody as well as vaccine discovery, an exclusive or non-exclusive license must be set up.