Purified Membrane Proteins

A2A GPCR Adenosine Receptors Class A

CALIXAR’s A2A GPCR (Adenosine receptor A2A) aids in the discovery, development, and validation of new molecules as well as therapeutic antibodies. They are ideal for CNS neuroprotection in order to develop new treatments for insomnia, pain, depression, Alzheimer and Parkinson’s diseases.

A2A Adenosine receptors are powerful therapeutic targets especially found in the treatment of such conditions as insomnia, pain, depression, and Parkinson’s disease.

Purchase A2A Adenosine Receptors Class A GPCR

This class A GPCR is involved in the regulation of myocardial blood flow/hypertension. This protein has a N-terminal Step tag II and an 8X His tag followed by a TEV protease cleavage site.

Target name: Adenosine receptor (A2A receptor)

Catalogue number: PP1

Class: GPCR Class A

Sequence: Full-length, wildtype sequence, with a N-terminus Strep tag II, 8xHis-tag, and TEV
protease cleavage site.

Affinity Tag​: His/Strep (both N-terminal)​

Origin: Human (Homo sapiens)​

Theor. MW: 47,7kDa

Accession #: P29274 (UniProt)​

Shipment temperature: Dry Ice

Storage conditions: Store at -80°C​

Expression system: Sf9 insect cells (baculovirus)​

Purity: >90%

Purification: Immobilized Metal Affinity Chromatography

Activity: Confirmed by radiobinding assay

Concentration​: Up to 5mg/ml

Sample buffer: 50mM Hepes pH 7.4, 200mM NaCl, 0.05%/0.006% DDM/CHS

Available quantity: From 10µg up to mg scale

€1,000.00 (10µg)

ships in 7 business days

First in market

CALIXAR is the first to produce native, wild-type, unmutated and untruncated targets.

Better discovery

Develop reliable and effective drugs and antibodies as they are not locked in any specific conformation.

Why choose us?

Adenosine A2A Receptors : We use a unique & custom-built approach to give us the edge

CALIXAR’s A2A GPCR Adenosine receptors facilitate reliable fragment-based drug design (FBDD), structure-based drug discovery (SBDD) and antibody discovery corresponding to this specific target. 

Unlike CALIXAR’s A2A Adenosine receptors, other alternative methods result in an adenosine receptor that becomes mutated and truncated (96 amino-acids truncation in the C-terminus). Additionally, this mutated version becomes locked within an antagonist conformation.

As with all GPCRs, the A2A Adenosine receptor is an unstable target and is difficult to produce natively with conventional approaches. Previously, A2A Adenosine receptors could only be locked in one specific conformation (e.g. antagonist) and were only ever prepared in a solution that inherently could not be pure, nor native (truncated, mutated) and without PTMs. A2A’s have only ever been inherently unstable, but not anymore.

Today, CALIXAR’s A2A receptors are able to bind to agonists, antagonists, as well as allosteric modulators (Igonet S. et al., 2018, Scientific Reports). Our A2A receptors also maintain their structural and functional integrity and are purified and stabilized to full length and wild-type (native) proteins.

CALIXAR’s A2A Adenosine receptor is the first native full-length and functional target on the market. Other existing A2A targets are either mutated or truncated. Our A2A protein is produced in a eukaryotic system with the proper post-translational modifications (glycosylation).

CALIXAR’s A2A Adenosine receptors are high-quality membrane proteins used for (bio)drug discovery and are adapted for use in pharmaceutical, biotechnology companies, and as well as for academic teams that are involved in the life science fields.

  • Antibodies (including nanobodies, scaffold proteins, aptamers)
  • Small molecules
  • 3D Structures (classical X-ray or XFEL, Cryo-EM, NMR)
  • Drug discovery (Screening: HTS, FBDD, SBDD; Hit and lead validation)
  • Antibody discovery (Immunization and display technologies)
  • Clinical stage (drug validation on reliable native A2A)​

Adenosine A2A GPCR Receptor References

Scientific Reports

Enabling STD-NMR fragment screening using stabilized native GPCR : A case study of adenosine receptor

Igonet S et al. 2018

Discovery on Target

Towards Native and Stable GPCRs for Conformational Antibody Development

Jawhari A. Boston, 2015 

ANALYTICAL BIOCHEMISTRY

Novel systematic detergent screening method for membrane proteins solubilization.

Desuzinges Mandon E. et al. 2017

Membrane Protein Purchase

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Or leave us an email : contact(at)calixar.com