Membrane Protein Assays
Thermal Stability Assays

CALIXAR proposes Thermal Stability Assays to get information about expression levels, stability and ligand binding of membrane proteins. The Andromeda system (Nanotemper) gives protein thermal unfolding data to help scientists to define optimal expression conditions that ensure the highest expression level together with the best thermal stability of membrane proteins.

Protein Characterization with Thermal Stability Assays

Access to the latest technology of Thermal Stability Assays using Andromeda system (Nanotemper).

Andromeda measures the thermal unfolding of a protein by detecting changes in fluorescence during a thermal ramp.

Sample volume

10 μl

Samples analyzed per run

Up to 48

Temperature range

15 - 95 °C

Requirement

Polyhistine Tag
GFP Tag

Protein sample

Cell lysate
Cell membrane
Purified protein
Muscarinic Acetylcholine Receptor M4-GFP in HEK cells

Expression level and thermal-stability measurement of M4-GFP during a time course of expression in HEK cell lysate.

M4-GFP

Ti (°C)

24h

50.18 ± 0.16

48h

50.78 ± 0.20

72h

51.49 ± 0.94
Purified Adenosine A2A Receptor

Thermal-stability measurement of A2AR purified in different detergent conditions in presence of ligands.

CALX10/DDM-CHS
DDM-CALXlc41

Ti (°C)

CALX 10/DDM-CHS

DDM-CALXlc41

Apo

38.93 ± 0.7

40.60 ± 0.1

+ CGS-21680

(agonist)

48.74 ± 0.1

49.63 ± 0.1

+ ZM-241385

(inverse agonist)

44.48 ± 0.1

46.32 ± 0.0

Get access to CALIXAR's Membrane Protein Assays Technology

All of our clients receive access to our proprietary technology and know-how (8 patent families, more than 28 publications).

The CALIXAR® platform uses patented technology only possible for clients under explicit agreements (license, co-development, and services).

You can leverage CALIXAR’s proprietary technology as well as other leading-in-class membrane protein technology that is not found anyplace else on the market.

Gain access to our specific expertise with a strong track record and several types of membrane proteins. We are continuously integrating best-of-the-art membrane protein characterization technology for our clients’ projects. Our clients have access to all assays needed for their projects, all on one platform.

  • Save time and money by using our unique assay services all in the same place.
  • Possibility to develop customized assays (transferable to our clients).
  • Possibility to validate customers’ hits/leads and the quality of the proteins produced.