CALIXAR proposes Thermal Stability Assays to get information about expression levels, stability and ligand binding of membrane proteins. The Andromeda system (Nanotemper) gives protein thermal unfolding data to help scientists to define optimal expression conditions that ensure the highest expression level together with the best thermal stability of membrane proteins.
Access to the latest technology of Thermal Stability Assays using Andromeda system (Nanotemper).
Andromeda measures the thermal unfolding of a protein by detecting changes in fluorescence during a thermal ramp.
Sample volume | 10 μl |
Samples analyzed per run | Up to 48 |
Temperature range | 15 - 95 °C |
Requirement | Polyhistine Tag GFP Tag |
Protein sample | Cell lysate Cell membrane Purified protein |
Expression level and thermal-stability measurement of M4-GFP during a time course of expression in HEK cell lysate.
M4-GFP | Ti (°C) |
24h | 50.18 ± 0.16 |
48h | 50.78 ± 0.20 |
72h | 51.49 ± 0.94 |
Thermal-stability measurement of A2AR purified in different detergent conditions in presence of ligands.
Ti (°C) | CALX 10/DDM-CHS | DDM-CALXlc41 |
Apo | 38.93 ± 0.7 | 40.60 ± 0.1 |
+ CGS-21680 (agonist) | 48.74 ± 0.1 | 49.63 ± 0.1 |
+ ZM-241385 (inverse agonist) | 44.48 ± 0.1 | 46.32 ± 0.0 |
All of our clients receive access to our proprietary technology and know-how (8 patent families, more than 28 publications).
The CALIXAR® platform uses patented technology only possible for clients under explicit agreements (license, co-development, and services).
You can leverage CALIXAR’s proprietary technology as well as other leading-in-class membrane protein technology that is not found anyplace else on the market.
Gain access to our specific expertise with a strong track record and several types of membrane proteins. We are continuously integrating best-of-the-art membrane protein characterization technology for our clients’ projects. Our clients have access to all assays needed for their projects, all on one platform.
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Starting from native material or recombinant systems, we succeed with all types of membrane proteins: GPCRs, Ions Channels, Transporters, Receptors and Viral Proteins.
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