Anass JAWHARI – CSO of CALIXAR – will be presenting collaborative work with THERMOFISHER SCIENTIFIC on September 25th 2019 at 08:00 AM PDT (5:00 PM in France).
This talk will focus on strategies for optimizing expression levels of membrane proteins using the Gibco™ Expi293™ and ExpiCHO™ Expression Systems. Additionally, there will be an introduction to newly developed Expi293 GNTI- and inducible cell lines, which can be combined with CALIXAR’s patented technology, to improve quality and quantity of challenging membrane proteins. A case study of potassium chloride co-transporter (KCC2) production and characterization will be further described.
Learning Objectives:
- Understand techniques to optimize expression levels of membrane proteins
- Understand how to improve quality and quantity of challenging membrane proteins using Expi293 GNTI- and inducible cell lines
