Histone H3
10 µg of active Histone H3. N-terminal 6His-tagged, recombinant, human Histone H3 amino acids 2-end.
Biological information
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Aug 2015]
Target class
Kinase
Family
Accession number
BC031333.1
Target Name
Histone H3
Target Alias
H3F3A; H3F3;H3F3B
Origin
Human
Theori. MW
17.5 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed in E. coli cells
Purity
95% by SDS-PAGE and Coomassie blue staining
Purification method
Immobilised metal affinity chromatography
Sample Buffer
10 mM Sodium phosphate buffer, pH 7.4, 130 mM NaCl, 10% glycerol, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Chemical data
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Aug 2015]
Compound name
Kinase
Catalog number
12-800
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
12-800
Datasheet