Akt1/PKBα, active
N-terminal 6His-tagged recombinant, human full length Akt1.
No items found.
Biological information
Background
PKB (also known as c-Akt or Rac-PK) was originally identified as the transforming oncogene in a retrovirus from a spontaneous thymoma in an AKR mouse. PKB appears to be activated as a consequence of increased phosphoinositide 3-kinase (PI3K) activity in cells stimulated with mitogens. Potential targets of PKB include glycogen synthase kinase-3 and p70 ribosomal protein S6 kinase. PKB is also crucial in mediating cell survival. Activation forms of both PI3 kinase and PKB have been shown to protect from apoptosis induced by withdrawal of growth and survival factors, expression of c-myc, and detachment from extracellular matrix.
GenBank M63167. The recombinant protein contains the amino acid substitution S478G with respect to this accession number. This is reported inEMBL BE 206796.
Target class
Kinase
Family
AGC
Accession number
NM_001014431.1; NM_001014432.1; NM_005163.2
Target Name
Akt1/PKBα, active
Target Alias
AKT1, RAC, PRKBA, MGC99656, RAC-ALPHA, RAC-PK-alpha, C-AKT, PKB, AKT
Origin
Human
Theori. MW
59.9 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Glutathione agarose affinity chromatography; Ni2+/NTA agarose
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 150mM NaCl, 150mM imidazole, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.07% 2-mercaptoethanol, 0.02% Brij 35, 270mM sucrose. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
PKB (also known as c-Akt or Rac-PK) was originally identified as the transforming oncogene in a retrovirus from a spontaneous thymoma in an AKR mouse. PKB appears to be activated as a consequence of increased phosphoinositide 3-kinase (PI3K) activity in cells stimulated with mitogens. Potential targets of PKB include glycogen synthase kinase-3 and p70 ribosomal protein S6 kinase. PKB is also crucial in mediating cell survival. Activation forms of both PI3 kinase and PKB have been shown to protect from apoptosis induced by withdrawal of growth and survival factors, expression of c-myc, and detachment from extracellular matrix.
GenBank M63167. The recombinant protein contains the amino acid substitution S478G with respect to this accession number. This is reported inEMBL BE 206796.
Compound name
Kinase
Catalog number
14-276
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-276
Datasheet