Akt1/PKBα, unactive
N-terminal 6His-tagged recombinant, human full length Akt1.
No items found.
Biological information
Background
Akt1 is an oncogenic AGC kinase that plays a critical role in regulating many processes including cell survival, metabolism, proliferation, growth and angiogenesis. AKT activation relies on the PI3K pathway, and is recognized as a critical node in the pathway. The E17 hotspot is the most characterized of AKT1 mutations, and has been shown to result in activation of the protein. Mutations in AKT1 have also been shown to confer resistance to allosteric kinase inhibitors in vitro.
GenBank M63167. The recombinant protein contains the amino acid substitution S478G with respect to this accession number. This conflict is reported inEMBL BE 206796. The residue coordinates in the native sequence are given.
Target class
Kinase
Family
AGC
Accession number
NM_001014431.1; NM_001014432.1; NM_005163.2
Target Name
Akt1/PKBα, unactive
Target Alias
AKT1, RAC, PRKBA, MGC99656, RAC-ALPHA, RAC-PK-alpha, C-AKT, PKB, AKT
Origin
Human
Theori. MW
59 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -20°C
Usage disclaimer
For Research Only
Chemical data
Background
Akt1 is an oncogenic AGC kinase that plays a critical role in regulating many processes including cell survival, metabolism, proliferation, growth and angiogenesis. AKT activation relies on the PI3K pathway, and is recognized as a critical node in the pathway. The E17 hotspot is the most characterized of AKT1 mutations, and has been shown to result in activation of the protein. Mutations in AKT1 have also been shown to confer resistance to allosteric kinase inhibitors in vitro.
GenBank M63167. The recombinant protein contains the amino acid substitution S478G with respect to this accession number. This conflict is reported inEMBL BE 206796. The residue coordinates in the native sequence are given.
Compound name
Kinase
Catalog number
14-279
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-279
Datasheet