Akt2/PKBβ (δPH, S474D), active
N-terminal 6His-tagged recombinant, human PKBβ, amino acids 120-481, S474D; activated by PDK1.
No items found.
Biological information
Background
Akt2, also known as Protein Kinase B Beta, is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. Akt2 serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.
EMBL M95936. The recombinant protein contains an amino acid substitution, S474D, with reference to the native sequence. This mimics the phosphorylation of S474.
Target class
Kinase
Family
AGC
Accession number
NM_001626.3
Target Name
Akt2/PKBβ (δPH, S474D), active
Target Alias
AKT2, RAC-BETA, PRKBB, PKBBETA, RAC-PK-beta
Origin
Human
Theori. MW
42.8 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose & heparin-Sepharose™ chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Akt2, also known as Protein Kinase B Beta, is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. Akt2 serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.
EMBL M95936. The recombinant protein contains an amino acid substitution, S474D, with reference to the native sequence. This mimics the phosphorylation of S474.
Compound name
Kinase
Catalog number
14-339
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-339
Datasheet