Cdk1/cyclin B, active
C-terminal 6His-tagged recombinant, human full length cdk1, and N-terminal GST-tagged human full length cyclin B.
No items found.
Biological information
Background
The cyclin-dependent kinases (CDKs), a subfamily of protein kinases, promote cell cycle progression. In mammals, nine different CDKs (cdk 1 to cdk 9; cdk 1 is better known as cdc 2) have been identified. CDKs are regulated on various levels including binding of proteins (cyclins, inhibitors, and assembly factors), protein degradation, transcriptional control, localization, and multiple phosphorylations. This leads to timely regulation of CDK activity, allowing progression from one cell cycle phase to the next. The cdk1 induces entry into M-phase of the cell cycle in eukaryotes.
GenBank NM_001786 / EMBL M25753
Target class
Kinase
Family
CMGC
Accession number
NM_001786.2; NM_033379.2
Target Name
Cdk1/cyclin B, active
Target Alias
CDC2, CDK1, MGC111195, DKFZp686L20222, CDC28A
Origin
Human
Theori. MW
35 kDa (cdk1), 75 kDa (cyclin B)
Affinity tag
GST, 6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose; GST-agarose. The cdk1 is then activated using CAK and repurified by Q Sepharose™ and Ni2+/NTA-agarose.
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
The cyclin-dependent kinases (CDKs), a subfamily of protein kinases, promote cell cycle progression. In mammals, nine different CDKs (cdk 1 to cdk 9; cdk 1 is better known as cdc 2) have been identified. CDKs are regulated on various levels including binding of proteins (cyclins, inhibitors, and assembly factors), protein degradation, transcriptional control, localization, and multiple phosphorylations. This leads to timely regulation of CDK activity, allowing progression from one cell cycle phase to the next. The cdk1 induces entry into M-phase of the cell cycle in eukaryotes.
GenBank NM_001786 / EMBL M25753
Compound name
Kinase
Catalog number
14-450
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-450
Datasheet