Cdk2/Cyclin E, active
Complex of recombinant, human full length cdk2 containing a C-terminal 6His-tag, and recombinant, human full length cyclin E containing an N-terminal GST-tag.
No items found.
Biological information
Background
The cyclin-dependent kinases (CDKs), are a family of serine threonine protein kinases, that control the cell division cycle. In mammals, nine different CDKs (cdk 1 to cdk 9) have been identified. CDKs are regulated on various levels including binding of proteins (cyclins, inhibitors, and assembly factors), protein degradation, transcriptional control, localization, and multiple phosphorylations. This leads to timely regulation of CDK activity, allowing progression from one cell cycle phase to the next. The cdk2 promotes the G1/S trasition and progression through the S phase when bound to specific cyclins (E and A). Recent evidences indicate that CDK2 is involved in cell cycle independent functions such as DNA damage repair.
EMBL M68520 / GenBank NM_001238
Target class
Kinase
Family
CMGC
Accession number
NM_001798.2; NM_052827.1
Target Name
Cdk2/Cyclin E, active
Target Alias
CDK2, p33(CDK2)
Origin
Human
Theori. MW
34 kDa (cdk2), 74 kDa (cyclin)
Affinity tag
GST, 6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
The cyclin-dependent kinases (CDKs), are a family of serine threonine protein kinases, that control the cell division cycle. In mammals, nine different CDKs (cdk 1 to cdk 9) have been identified. CDKs are regulated on various levels including binding of proteins (cyclins, inhibitors, and assembly factors), protein degradation, transcriptional control, localization, and multiple phosphorylations. This leads to timely regulation of CDK activity, allowing progression from one cell cycle phase to the next. The cdk2 promotes the G1/S trasition and progression through the S phase when bound to specific cyclins (E and A). Recent evidences indicate that CDK2 is involved in cell cycle independent functions such as DNA damage repair.
EMBL M68520 / GenBank NM_001238
Compound name
Kinase
Catalog number
14-475
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-475
Datasheet