GSK3α, active
N-terminal 6His-tagged recombinant, human GSK3α amino acids 2-end.
No items found.
Biological information
Background
Glycogen synthase kinase 3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. There exists two isoforms of GSK3, GSK3A and GSK3B, and they are strictly regulated via phosphorylation. GSK3 has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3A also has been inhibited by activation of the canonical Wnt pathway, resulting in increased catenin that drives human mesenchymal stem cells to osteoblasts.
GenBank NM_019884. The recombinant GSK3a sequence deviates from that published in NM_019884 by a single amino acid substitution, S449A. This substitution has been reported elsewhere,GenBank 2641993.
Target class
Kinase
Family
CMGC
Accession number
NM_019884.2
Target Name
GSK3α, active
Target Alias
GSK3A, DKFZp686D0638
Origin
Human
Theori. MW
55 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Glycogen synthase kinase 3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. There exists two isoforms of GSK3, GSK3A and GSK3B, and they are strictly regulated via phosphorylation. GSK3 has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3A also has been inhibited by activation of the canonical Wnt pathway, resulting in increased catenin that drives human mesenchymal stem cells to osteoblasts.
GenBank NM_019884. The recombinant GSK3a sequence deviates from that published in NM_019884 by a single amino acid substitution, S449A. This substitution has been reported elsewhere,GenBank 2641993.
Compound name
Kinase
Catalog number
14-492
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-492
Datasheet