Cdk6/Cyclin D3, active
N-terminal 6His-tagged recombinant, human full length cdk6 complexed with N-terminal GST-tagged full length human cyclin D3.
No items found.
Biological information
Background
Mammalian cells express two structurally redundant kinases (Cdk4 and Cdk6) and three structurally redundant partner cyclins (D1, D2 and D3), with predominant expressions of Cdk4 and D1 in mesenchymal cells and Cdk6 and D3 in hematopietic cells. Cdk6 is though to be functionally redundant with Cdk4 and to play the same role in hematopoietic cells as does Cdk4 in fibroblasts. Cyclin D3 is widely expressed in a variety of cells and has been suggested to play a role in the G1-S transition of hematopoietic cells and fibroblasts. Furthermore, recent studies have implicated the involvement of this cyclin subtype in a variety of decisions on cell fate, including proliferation, apoptosis and differentiation.
GenBank X66365 / EMBL M90814
Target class
Kinase
Family
CMGC
Accession number
NM_001259.5
Target Name
Cdk6/Cyclin D3, active
Target Alias
CDK6, PLSTIRE, MGC59692
Origin
Human
Theori. MW
38 kDa (cdk6), 59 kDa (cyclin D3)
Affinity tag
GST, 6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Glutathione agarose affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Mammalian cells express two structurally redundant kinases (Cdk4 and Cdk6) and three structurally redundant partner cyclins (D1, D2 and D3), with predominant expressions of Cdk4 and D1 in mesenchymal cells and Cdk6 and D3 in hematopietic cells. Cdk6 is though to be functionally redundant with Cdk4 and to play the same role in hematopoietic cells as does Cdk4 in fibroblasts. Cyclin D3 is widely expressed in a variety of cells and has been suggested to play a role in the G1-S transition of hematopoietic cells and fibroblasts. Furthermore, recent studies have implicated the involvement of this cyclin subtype in a variety of decisions on cell fate, including proliferation, apoptosis and differentiation.
GenBank X66365 / EMBL M90814
Compound name
Kinase
Catalog number
14-519
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-519
Datasheet