B-Raf (V599E), active
N-terminal GST-tagged recombinant, human B-Raf amino acids 416-end, containing a V599E mutation (also designated V600E).
No items found.
Biological information
Background
B-RAF plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes.
GenBank NM_004333. The V599E mutation is thought to mimic phosphorylation of the native enzyme, resulting in a protein with high activity and leading to constitutive ERK activation. As B-Raf is commonly activated by somatic point mutation in human cancer, it may provide a new therapeutic approach to malignant melanoma. (Davies H., et al., Nature, 2002. 417: 949-54, and Mercer KE., & Pritchard CA., Biochim Biophys Acta. 2003. 1653: 25-40)
Target class
Kinase
Family
TKL
Accession number
NM_004333.3
Target Name
B-Raf (V599E), active
Target Alias
BRAF, BRAF1, B-Raf, MGC126806, RAFB1, p94, MGC138284, B-raf-1, BRAF V600E, B-RAF V600E
Origin
Human
Theori. MW
67.3 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Glutathione agarose affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
B-RAF plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes.
GenBank NM_004333. The V599E mutation is thought to mimic phosphorylation of the native enzyme, resulting in a protein with high activity and leading to constitutive ERK activation. As B-Raf is commonly activated by somatic point mutation in human cancer, it may provide a new therapeutic approach to malignant melanoma. (Davies H., et al., Nature, 2002. 417: 949-54, and Mercer KE., & Pritchard CA., Biochim Biophys Acta. 2003. 1653: 25-40)
Compound name
Kinase
Catalog number
14-557
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-557
Datasheet