LMPTP-B
N-terminal GST-tagged recombinant, human full length, human LMPTP-B.
Biological information
Background
LMPTP-B (Low Molecular Weight Protein Tyrosine Phosphatase B) is a type of protein phosphatase that specifically targets tyrosine residues in proteins. Phosphatases are enzymes that catalyze the removal of phosphate groups from proteins, and this dephosphorylation event can regulate the activity, localization, and interactions of proteins in a cell. The primary function of LMPTP-B is to dephosphorylate tyrosine residues in proteins that are involved in cell signaling pathways. This includes receptor tyrosine kinases (RTKs), which are a class of cell surface receptors that activate signaling pathways when they bind to ligands, and cytoplasmic tyrosine kinases such as Src, which are involved in downstream signaling pathways. Through dephosphorylation of tyrosine residues, LMPTP-B can modulate various cellular processes, including cell proliferation, differentiation, and survival.
Target class
Phosphatase
Family
Tyr phosphatase
Accession number
NM_007099
Target Name
LMPTP-B
Target Alias
Origin
Human
Theori. MW
44 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed in E. coli
Purity
Purity 100% by SDS-PAGE and Coomassie blue staining
Purification method
Glutathione agarose affinity chromatography
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Chemical data
Background
LMPTP-B (Low Molecular Weight Protein Tyrosine Phosphatase B) is a type of protein phosphatase that specifically targets tyrosine residues in proteins. Phosphatases are enzymes that catalyze the removal of phosphate groups from proteins, and this dephosphorylation event can regulate the activity, localization, and interactions of proteins in a cell. The primary function of LMPTP-B is to dephosphorylate tyrosine residues in proteins that are involved in cell signaling pathways. This includes receptor tyrosine kinases (RTKs), which are a class of cell surface receptors that activate signaling pathways when they bind to ligands, and cytoplasmic tyrosine kinases such as Src, which are involved in downstream signaling pathways. Through dephosphorylation of tyrosine residues, LMPTP-B can modulate various cellular processes, including cell proliferation, differentiation, and survival.
Compound name
Phosphatase
Catalog number
14-620
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-620
Datasheet