DAPK2, active
N-terminal GST-tagged recombinant, human full length DAPK2.
No items found.
Biological information
Background
Death-associated protein kinase (DAPK) is a calcium/calmodulin-dependent kinase that phosphorylates serine/threonine residues on proteins. DAPK modulates cell death induced by IFN-g, TNF-a, Fas, and detachment from the extracellular matrix. DAPK has been also implicated in TGF-binduced apoptosis in several cultured cell lines in which Smad proteins mediate transcriptional activation of DAPK. A member of DAP kinase family, DAPK2 is a 42 KDa calcium/calmodulin-dependant serine/threonine kinase localized in the cytoplasm. Reports suggest DAPK2 along with DAPK1 induces membrane blebbing and formation of autophagic vesicles and plays a vital role in TGF-induced caspase-dependent Type-I and IFNg-induced caspase-independent autophagic type II cell death signaling. Inhibition of DAPK2 may facilitate tumor progression.
GenBank NM_014326
Target class
Kinase
Family
CAMK
Accession number
NM_014326.3
Target Name
DAPK2, active
Target Alias
DAPK2, DRP-1, MGC119312
Origin
Human
Theori. MW
70.6 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Death-associated protein kinase (DAPK) is a calcium/calmodulin-dependent kinase that phosphorylates serine/threonine residues on proteins. DAPK modulates cell death induced by IFN-g, TNF-a, Fas, and detachment from the extracellular matrix. DAPK has been also implicated in TGF-binduced apoptosis in several cultured cell lines in which Smad proteins mediate transcriptional activation of DAPK. A member of DAP kinase family, DAPK2 is a 42 KDa calcium/calmodulin-dependant serine/threonine kinase localized in the cytoplasm. Reports suggest DAPK2 along with DAPK1 induces membrane blebbing and formation of autophagic vesicles and plays a vital role in TGF-induced caspase-dependent Type-I and IFNg-induced caspase-independent autophagic type II cell death signaling. Inhibition of DAPK2 may facilitate tumor progression.
GenBank NM_014326
Compound name
Kinase
Catalog number
14-657
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-657
Datasheet