DCAMKL2, active
N-terminal 6His-tagged recombinant, human DCAMKL2 amino acids 376-end.
No items found.
Biological information
Background
DCAMKL2 is a protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. It may play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm (By similarity).
Target class
Kinase
Family
CAMK
Accession number
NM_001040260.1; NM_001040261.1
Target Name
DCAMKL2, active
Target Alias
DCLK2, DKFZp761I032, DCDC3B, DCDC3, DCK2, DCAMKL2, MGC45428
Origin
Human
Theori. MW
39.1 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 86% by SDS-PAGE and Coomassie blue staining.
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50mM Tris/HCl pH7.5,
300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose,
1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
DCAMKL2 is a protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. It may play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm (By similarity).
Compound name
Kinase
Catalog number
14-716
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-716
Datasheet