CHK2 (I157T), active
10 µg of active CHK2. N-terminal GST and C-terminal 6His-tagged recombinant, human CHK2, amino acids 5-end, containing the I157T mutation.
No items found.
Biological information
Background
Checkpoint kinase (CHK) is activated by a broad spectrum of DNA-damaging agents, including DNA-strand-breaking agents such as ionizing radiation and topoisomerase inhibitors, and those agents that cause replication stress such as ultraviolet light, hydroxyurea and 5-fluorouracil. CHK2 might have a redundant and supportive role in checkpoints, but its role in ionizing radiation-induced apoptosis is more prominent. However, its role in other DNA-damage-induced apoptosis mechanisms is less well established. CHK2 inhibitors are expected to sensitize proliferating cells to chemotherapy-induced apoptosis.
GenBank NM 007194
Target class
Kinase
Family
CAMK
Accession number
NM_007194
Target Name
CHK2 (I157T), active
Target Alias
CHEK2, CHK2, RAD53, CDS1, LFS2
Origin
Human
Theori. MW
89.6 kDa
Affinity tag
GST, 6His
Product specifications
Expression system
Expressed in E. coli
Purity
Refer to CoA for Purity
Purification method
Glutathione agarose affinity chromatography; Ni2+/NTA agarose
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Checkpoint kinase (CHK) is activated by a broad spectrum of DNA-damaging agents, including DNA-strand-breaking agents such as ionizing radiation and topoisomerase inhibitors, and those agents that cause replication stress such as ultraviolet light, hydroxyurea and 5-fluorouracil. CHK2 might have a redundant and supportive role in checkpoints, but its role in ionizing radiation-induced apoptosis is more prominent. However, its role in other DNA-damage-induced apoptosis mechanisms is less well established. CHK2 inhibitors are expected to sensitize proliferating cells to chemotherapy-induced apoptosis.
GenBank NM 007194
Compound name
Kinase
Catalog number
14-741
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-741
Datasheet