Haspin, active
N-terminal 6His-tagged recombinant, human Haspin amino acids 471-end. Histone and Histone-Modifying Enzyme.
No items found.
Biological information
Background
HASPIN (Histone H3 Associated Protein Kinase) is a Protein Coding gene. An important paralog of this gene is IRAK1. This protein is a Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. May act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.
GenBank NM_031965
Target class
Kinase
Family
Other
Accession number
NM_031965.2
Target Name
Haspin, active
Target Alias
GSG2, H-Haspin, haspin
Origin
Human
Theori. MW
41.1 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 83% by SDS-PAGE and Coomassie blue staining.
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
HASPIN (Histone H3 Associated Protein Kinase) is a Protein Coding gene. An important paralog of this gene is IRAK1. This protein is a Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. May act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.
GenBank NM_031965
Compound name
Kinase
Catalog number
14-744
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-744
Datasheet