Abl (Q252H), active
10 µg of active Abl (Q252H). N-terminal 6His-tagged recombinant, human Abl, amino acids 27-end containing the Q252H mutation.
No items found.
Biological information
Background
N-terminal 6His-tagged recombinant, human Abl, amino acids 27-end containing the Q252H mutation. The Abl tyrosine kinase inhibitor STI571 is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the Q252H mutation, which confers resistance to drug binding (Shah et al., (2002), Cancer Cell 2, 117-125).
Target class
Kinase
Family
TK
Accession number
NM_005157.3; NM_007313.2
Target Name
Abl (Q252H), active
Target Alias
ABL1, JTK7, p150, bcr/abl, v-abl, c-ABL, ABL
Origin
Human
Theori. MW
121 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 51% by SDS-PAGE and Coomassie staining.
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50 mM Tris/HCl pH7.5, 150 mM NaCl, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1 mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
N-terminal 6His-tagged recombinant, human Abl, amino acids 27-end containing the Q252H mutation. The Abl tyrosine kinase inhibitor STI571 is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the Q252H mutation, which confers resistance to drug binding (Shah et al., (2002), Cancer Cell 2, 117-125).
Compound name
Kinase
Catalog number
14-751
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-751
Datasheet