GRK7, active
N-terminal 6His-tagged recombinant, human full length GRK7.
No items found.
Biological information
Background
GRK7 (G Protein-Coupled Receptor Kinase 7) is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. It is specifically expressed in the retina and the encoded protein has been shown to phosphorylate cone opsins and initiate their deactivation. It involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).
GenBank NM_139209
Target class
Kinase
Family
AGC
Accession number
NM_139209.2
Target Name
GRK7, active
Target Alias
GRK7, GPRK7
Origin
Human
Theori. MW
66 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 99% by SDS-PAGE and Coomassie blue staining.
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50 mM Tris-HCl pH 8.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
GRK7 (G Protein-Coupled Receptor Kinase 7) is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. It is specifically expressed in the retina and the encoded protein has been shown to phosphorylate cone opsins and initiate their deactivation. It involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).
GenBank NM_139209
Compound name
Kinase
Catalog number
14-752
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-752
Datasheet