Abl (Y253F), active
10 µg of active Abl (Y253F). N-terminal 6His-tagged recombinant, human Abl, amino acids 27-end containing the Y253F mutation.
No items found.
Biological information
Background
N-terminal 6His-tagged recombinant, human Abl, amino acids 27-end containing the Y253F mutation. The Abl tyrosine kinase inhibitor STI571 is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the Y253F mutation, which confers resistance to drug binding (Shah et al., (2002), Cancer Cell 2, 117-125).
Target class
Kinase
Family
TK
Accession number
U07563
Target Name
Abl (Y253F), active
Target Alias
ABL1, JTK7, ABL, p150, vABL, cABL, bcr/abl
Origin
Human
Theori. MW
121 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 150mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij 35. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
N-terminal 6His-tagged recombinant, human Abl, amino acids 27-end containing the Y253F mutation. The Abl tyrosine kinase inhibitor STI571 is an effective therapy for stable-phase chronic myeloid leukemia patients. Many patients responding to STI571 later relapse due to a reactivation of Bcr-Abl activity. In certain cases this appears to correlate with the presence of the Y253F mutation, which confers resistance to drug binding (Shah et al., (2002), Cancer Cell 2, 117-125).
Compound name
Kinase
Catalog number
14-759
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-759
Datasheet