CLK2, active
N-terminal GST-tagged recombinant, human CLK2 amino acids 138-end.
No items found.
Biological information
Background
CLK2 (CDC2-like kinases) proteins contain an Serine- and Arginine-rich (SR) domain and autophosphorylate on tyrosine, serine, and threonine residues in overexpression systems and in vitro. Mutations in the clk-2 gene of the nematode Caenorhabditis elegans affect organismal features such as development, behavior, reproduction, and aging as well as cellular features such as the cell cycle, apoptosis, the DNA replication checkpoint, and telomere length. These results suggest the existence of a novel class of protein kinases, with an unusual substrate specificity, which may be involved in cell cycle control. Overexpression of CLK2 decreases cell cycle length. Inhibition of CLK2 may reversibly arrest the cell cycle and tumor growth.
GenBank NM_003993
Target class
Kinase
Family
CMGC
Accession number
NM_003993
Target Name
CLK2, active
Target Alias
CLK2
Origin
Human
Theori. MW
70.4 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Glutathione agarose affinity chromatography
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
CLK2 (CDC2-like kinases) proteins contain an Serine- and Arginine-rich (SR) domain and autophosphorylate on tyrosine, serine, and threonine residues in overexpression systems and in vitro. Mutations in the clk-2 gene of the nematode Caenorhabditis elegans affect organismal features such as development, behavior, reproduction, and aging as well as cellular features such as the cell cycle, apoptosis, the DNA replication checkpoint, and telomere length. These results suggest the existence of a novel class of protein kinases, with an unusual substrate specificity, which may be involved in cell cycle control. Overexpression of CLK2 decreases cell cycle length. Inhibition of CLK2 may reversibly arrest the cell cycle and tumor growth.
GenBank NM_003993
Compound name
Kinase
Catalog number
14-774
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-774
Datasheet