MKP5, active
10 µg of active MKP5. N-terminal 6His-tagged recombinant, human MKP5 amino acids 320-end.
No items found.
Biological information
Background
MKP5, also known as DUSP10 (Dual Specificity Phosphatase 10), is a dual specificity protein phosphatase that inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. Dual specificity protein phosphatses negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK.
GenBank NM_007207
Target class
Phosphatase
Family
Accession number
NM_007207
Target Name
MKP5, active
Target Alias
DUSP10, MKP5
Origin
Human
Theori. MW
20.6 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed in E. coli
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Chemical data
Background
MKP5, also known as DUSP10 (Dual Specificity Phosphatase 10), is a dual specificity protein phosphatase that inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. Dual specificity protein phosphatses negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK.
GenBank NM_007207
Compound name
Phosphatase
Catalog number
14-779
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-779
Datasheet