AMPK (α1, β1, γ1), active
Recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 and full length untagged AMPKγ1.
No items found.
Biological information
Background
AMPK (α1, β1, γ1) protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
GenBank AF100763 / GenBank NM_006253 / GenBank NM_002733
Target class
Kinase
Family
CAMK
Accession number
AF100763; NM_006253; NM_002733
Target Name
AMPK (α1, β1, γ1), active
Target Alias
PRKAB1, PRKAG1 , PRKAA1
Origin
Human
Theori. MW
64 kDa (AMPKα1), 30 kDa (AMPKß1), 38 kDa (AMPKγ1)
Affinity tag
6His
Product specifications
Expression system
Expressed in E. coli cells and activated in vivo
Purity
Refer to CoA for Purity
Purification method
Cobalt-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
AMPK (α1, β1, γ1) protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
GenBank AF100763 / GenBank NM_006253 / GenBank NM_002733
Compound name
Kinase
Catalog number
14-840
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-840
Datasheet