Hck, activated
N-terminal 6His-tagged recombinant, human Hck amino acids 230–497 .
No items found.
Biological information
Background
Hematopoietic cell kinase (HCK) is a member of Src protein-tyrosine kinase family and expressed primary in granulocytes, monocytes and macrophages. Active HCK phosphorylates STAT5B on Tyr699, which represents an essential step in Signal transducer and activator of transcription 5 (STAT5B) stimulation. The binding of SH3 ligands, such as the Nef protein of HIV-1, further augments Hck activity suggesting a new mechanism for regulating tyrosine kinase activity.
GenBank NM_002110
Target class
Kinase
Family
TK
Accession number
NM_002110.2
Target Name
Hck, activated
Target Alias
HCK, p59-HCK/p60-HCK, JTK9
Origin
Human
Theori. MW
34.1 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 5mM β-glycerophosphate, 1mM Na3VO4. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Hematopoietic cell kinase (HCK) is a member of Src protein-tyrosine kinase family and expressed primary in granulocytes, monocytes and macrophages. Active HCK phosphorylates STAT5B on Tyr699, which represents an essential step in Signal transducer and activator of transcription 5 (STAT5B) stimulation. The binding of SH3 ligands, such as the Nef protein of HIV-1, further augments Hck activity suggesting a new mechanism for regulating tyrosine kinase activity.
GenBank NM_002110
Compound name
Kinase
Catalog number
14-843
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-843
Datasheet