JAK1, active
10 µg of active JAK1. N-terminal GST-tagged recombinant, human JAK1 amino acids 866-end. Activated in vitro by auto-phosphorylation.
No items found.
Biological information
Background
Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-α/beta and -γ signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-α pathway, and between JAK1 and JAK2 in the interferon-γ pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes.
Target class
Kinase
Family
TK
Accession number
NM_002227
Target Name
JAK1, active
Target Alias
JAK1, JTK3, JAK1A, JAK1B
Origin
Human
Theori. MW
61 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Glutathione Sepharose™ affinity chromatography
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 150mM NaCl, 5mM β-glycerophosphate, 0.5mM sodium orthovanadate, 50% v/v glycerol, 0.02% Triton® X-100, 0.5mM EDTA, 2mM DTT. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-α/beta and -γ signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-α pathway, and between JAK1 and JAK2 in the interferon-γ pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes.
Compound name
Kinase
Catalog number
14-918
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-918
Datasheet