LRRK2, active
10 µg of active LRRK2. N-terminal FLAG-tagged recombinant, human LRRK2 amino acids 970-end.
No items found.
Biological information
Background
This protein is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.
Target class
Kinase
Family
TKL
Accession number
BC117180
Target Name
LRRK2, active
Target Alias
LRRK2, PARK8, RIPK7, ROCO2, AURA17, DARDARIN
Origin
Human
Theori. MW
180 kDa
Affinity tag
FLAG
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Immunoaffinity chromatography
Sample Buffer
Protein in 50mM Tris/HCl pH7.4, 300mM NaCl, 20mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 150µg/ml FLAG peptide, 20% v/v glycerol, 1mM benzamidine, 0.2mM PMSF, 5mM DTT. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
This protein is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8.
Compound name
Kinase
Catalog number
14-919
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-919
Datasheet