IKKε, active
10 µg of active IKKε. N-terminal FLAG, GST-tagged recombinant, human IKKε full length.
No items found.
Biological information
Background
IKKe is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
Target class
Kinase
Family
Other
Accession number
NM_001193321.1
Target Name
IKKε, active
Target Alias
IKBKE, IKKe, IKKI, KIAA0151
Origin
Human
Theori. MW
109 kDa
Affinity tag
GST, FLAG
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Immunoaffinity chromatography
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 150mM NaCl, 10mM β-glycerophosphate, 1mM sodium orthovanadate, 1mM NaF, 28% v/v glycerol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
IKKe is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.
Compound name
Kinase
Catalog number
14-926
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-926
Datasheet