IRE1, active
10 µg of active IRE1. N-terminal 6His-tagged recombinant, human IRE1 amino acids 465-end.
No items found.
Biological information
Background
IRE1 (Inositol-requiring enzyme 1) is a transmembrane protein kinase that is involved in the endoplasmic reticulum (ER) stress response pathway. It is a key regulator of the unfolded protein response (UPR), which is activated in response to ER stress to maintain ER homeostasis and promote cell survival. Research has suggested that IRE1 plays a role in several diseases, including cancer, diabetes, and neurodegenerative diseases. In cancer, activation of IRE1 has been shown to promote cell survival and tumor growth, and inhibition of IRE1 has been suggested as a potential cancer therapy. In diabetes, activation of IRE1 has been implicated in the development of insulin resistance and beta cell dysfunction. Additionally, IRE1 has been implicated in the regulation of neuronal survival and degeneration, making it a potential target for the treatment of neurodegenerative diseases.
Target class
Kinase
Family
Ser/Thr Kinase
Accession number
NM_001433
Target Name
IRE1, active
Target Alias
ERN1, IRE1
Origin
Human
Theori. MW
62 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 93.2% by SDS-PAGE and Coomassie
blue staining.
Purification method
Purified using immobilized
metal affinity chromatography.
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
IRE1 (Inositol-requiring enzyme 1) is a transmembrane protein kinase that is involved in the endoplasmic reticulum (ER) stress response pathway. It is a key regulator of the unfolded protein response (UPR), which is activated in response to ER stress to maintain ER homeostasis and promote cell survival. Research has suggested that IRE1 plays a role in several diseases, including cancer, diabetes, and neurodegenerative diseases. In cancer, activation of IRE1 has been shown to promote cell survival and tumor growth, and inhibition of IRE1 has been suggested as a potential cancer therapy. In diabetes, activation of IRE1 has been implicated in the development of insulin resistance and beta cell dysfunction. Additionally, IRE1 has been implicated in the regulation of neuronal survival and degeneration, making it a potential target for the treatment of neurodegenerative diseases.
Compound name
Kinase
Catalog number
14-930
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-930
Datasheet