DYRK1A, active
N-terminal GST-tagged recombinant, human full length DYRK1A.
No items found.
Biological information
Background
DNA-PK is a Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. It involvesin DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination and must be bound to DNA to express its catalytic properties. It also promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion (By similarity). Also involved in modulation of transcription. As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome.
GenBank NM_006904.6
Target class
Kinase
Family
CMGC
Accession number
NM_130436.2
Target Name
DYRK1A, active
Target Alias
Origin
Human
Theori. MW
112 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 90% by SDS-PAGE and Coomassie blue staining.
Purification method
Glutathione agarose affinity chromatography; Gel filtration
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
DNA-PK is a Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. It involvesin DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination and must be bound to DNA to express its catalytic properties. It also promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion (By similarity). Also involved in modulation of transcription. As part of the DNA-PK complex, involved in the early steps of ribosome assembly by promoting the processing of precursor rRNA into mature 18S rRNA in the small-subunit processome.
GenBank NM_006904.6
Compound name
Kinase
Catalog number
14-951
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-951
Datasheet