A-Raf, active
N-terminal GST-tagged recombinant, human A-Raf amino acids 273-end containing the mutations Y301D and Y302D.
No items found.
Biological information
Background
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
GenBank U01337.1 The recombinant protein contains the amino acid substitution Y301D and Y302D with reference to GenBank U01337.1. These mutations provide constitutive activation of the enzyme.
Target class
Kinase
Family
TKL
Accession number
U01337.1
Target Name
A-Raf, active
Target Alias
Origin
Human
Theori. MW
65 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 58% by SDS-PAGE and Coomassie blue staining.
Purification method
Glutathione agarose affinity chromatography
Sample Buffer
Protein in 40mM Tris/HCl pH8.0, 200mM NaCl, 0.08mM EGTA, 0.024% Brij-35, 4mM glutathione, 20% (v/v) glycerol, 0.8mM benzamidine, 0.16mM PMSF, 0.08% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
GenBank U01337.1 The recombinant protein contains the amino acid substitution Y301D and Y302D with reference to GenBank U01337.1. These mutations provide constitutive activation of the enzyme.
Compound name
Kinase
Catalog number
14-956
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-956
Datasheet