CDK4/cyclin D3, active
N-terminal GST-tagged recombinant, human full length CDK4 and N-terminal GST-tagged recombinant, full length human cyclin D3.
No items found.
Biological information
Background
CDK4 (Cyclin-dependent kinase 4) forms a complex with type D cyclins such as D1, D2 and D3 and when the cellular levels of cyclin D peak there is a shortening of the G1 phase and rapid entry into S phase of cell cycle progression. The substrate for cdk4/cyclin D is Rb, and phosphorylation of Rb results in dissociation of the E2F complex from Rb. The free E2F proteins in turn stimulate transcription of genes required for DNA synthesis. Cellular inhibition of CDK4/cyclin D3 activity is achieved by its inhibitory proteins, i.e. p16, p21 and p27. Inhibition of CDK4/CCND3 may be used in treatment of uncontrolled cell proliferation, tumor heterogeneity, invasion and metastasis.
GenBank NM_000075.3 / GenBank M90814.1
Target class
Kinase
Family
CMGC
Accession number
NM_000075.3
Target Name
CDK4/cyclin D3, active
Target Alias
Origin
Human
Theori. MW
61 kDa (CDK4), 59 kDa (cyclin D3)
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 96% by SDS-PAGE and Coomassie blue staining.
Purification method
Glutathione agarose affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
CDK4 (Cyclin-dependent kinase 4) forms a complex with type D cyclins such as D1, D2 and D3 and when the cellular levels of cyclin D peak there is a shortening of the G1 phase and rapid entry into S phase of cell cycle progression. The substrate for cdk4/cyclin D is Rb, and phosphorylation of Rb results in dissociation of the E2F complex from Rb. The free E2F proteins in turn stimulate transcription of genes required for DNA synthesis. Cellular inhibition of CDK4/cyclin D3 activity is achieved by its inhibitory proteins, i.e. p16, p21 and p27. Inhibition of CDK4/CCND3 may be used in treatment of uncontrolled cell proliferation, tumor heterogeneity, invasion and metastasis.
GenBank NM_000075.3 / GenBank M90814.1
Compound name
Kinase
Catalog number
14-957
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-957
Datasheet