LATS2, active
N-terminal GST-tagged recombinant, human LATS2 amino acids 553-end and N-terminal 6His-tagged recombinant, human MOBKL1A full length.
No items found.
Biological information
Background
LATS2 is a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression.
GenBank NM_014572.2 / GenBank BC038112
Target class
Kinase
Family
AGC
Accession number
NM_014572.2; BC038112.1
Target Name
LATS2, active
Target Alias
Origin
Human
Theori. MW
89 kDa (LATS2), 30 kDa (MOBKL1A)
Affinity tag
GST, 6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 75% (combined) by SDS-PAGE and Coomassie blue staining.
Purification method
Glutathione agarose affinity chromatography
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
LATS2 is a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression.
GenBank NM_014572.2 / GenBank BC038112
Compound name
Kinase
Catalog number
14-987
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-987
Datasheet