HIPK4, active
C-terminal 6His tagged, recombinant, human HIPK4 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.
Biological information
Background
HIPK4 (Homeodomain Interacting Protein Kinase 4) is a serine/threonine protein kinase that belongs to the HIPK (Homeodomain Interacting Protein Kinase) family of kinases. It is involved in the regulation of a variety of cellular processes, including transcriptional regulation, cell proliferation, and apoptosis. Research has suggested that HIPK4 plays a role in several diseases, including cancer and developmental disorders. In cancer, HIPK4 has been shown to promote tumor cell survival and proliferation, and alterations in HIPK4 expression and activity have been observed in various types of cancer. Additionally, HIPK4 has been implicated in the regulation of neural development and has been linked to developmental disorders such as autism spectrum disorder. HIPK4 has also been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
Target class
Kinase
Family
CMGC
Accession number
BC034501.2
Target Name
HIPK4, active
Target Alias
Origin
Human
Theori. MW
71 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cell
Purity
Purity 78% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilized metal affinity chromatography
Sample Buffer
0.54 mg/ml of enzyme in 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
HIPK4 (Homeodomain Interacting Protein Kinase 4) is a serine/threonine protein kinase that belongs to the HIPK (Homeodomain Interacting Protein Kinase) family of kinases. It is involved in the regulation of a variety of cellular processes, including transcriptional regulation, cell proliferation, and apoptosis. Research has suggested that HIPK4 plays a role in several diseases, including cancer and developmental disorders. In cancer, HIPK4 has been shown to promote tumor cell survival and proliferation, and alterations in HIPK4 expression and activity have been observed in various types of cancer. Additionally, HIPK4 has been implicated in the regulation of neural development and has been linked to developmental disorders such as autism spectrum disorder. HIPK4 has also been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
Compound name
Kinase
Catalog number
15-003
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
15-003
Datasheet