CaMKK1, active
10 µg of active CaMKK1. N-terminal 6His-tagged, recombinant, human CaMKK1 full length.
Biological information
Background
The Calcium/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Calcium/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions. CaMKK1 plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
OTHER-CAMKK
Accession number
NM_172206.1
Target Name
CaMKK1, active
Target Alias
CAMKKA; CaM-KK Alpha; EC 2.7.11.17; Calcium/Calmodulin Dependent Protein Kinase Kinase 1
Origin
Human
Theori. MW
60 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 96% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
The Calcium/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Calcium/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions. CaMKK1 plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. [provided by RefSeq, Jul 2008]
Compound name
Kinase
Catalog number
15-022
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
15-022
Datasheet