ICK, active
N-terminal 6His-tagged, recombinant, human, amino acids 1-312 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.
Biological information
Background
ICK (intestinal cell kinase) is a serine/threonine protein kinase that is primarily expressed in the intestinal epithelium, where it plays a role in the regulation of cell proliferation and differentiation. ICK has been shown to be involved in the regulation of Wnt signaling, which is a critical pathway for the development and homeostasis of the intestinal epithelium. Research has suggested that ICK may also play a role in the development and progression of cancer. ICK has been shown to be overexpressed in several types of cancer, including colorectal cancer and pancreatic cancer, and high levels of ICK expression have been associated with poor prognosis. Additionally, ICK has been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
Target class
Kinase
Family
CMGC
Accession number
NM_014920.2
Target Name
ICK, active
Target Alias
Origin
Human
Theori. MW
40 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 69% by SDS-PAGE and Coomassie blue staining
Purification method
Immobilized metal affinity chromatography
Sample Buffer
0.25mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
ICK (intestinal cell kinase) is a serine/threonine protein kinase that is primarily expressed in the intestinal epithelium, where it plays a role in the regulation of cell proliferation and differentiation. ICK has been shown to be involved in the regulation of Wnt signaling, which is a critical pathway for the development and homeostasis of the intestinal epithelium. Research has suggested that ICK may also play a role in the development and progression of cancer. ICK has been shown to be overexpressed in several types of cancer, including colorectal cancer and pancreatic cancer, and high levels of ICK expression have been associated with poor prognosis. Additionally, ICK has been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
Compound name
Kinase
Catalog number
15-023
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
15-023
Datasheet