CDK18/cyclin Y, active
10 µg of active CDK18/cyclin Y. N-terminal GST-tagged, recombinant, human CDK18 full length and N-terminal GST-tagged, recombinant human cyclin Y full length.
Biological information
Background
CDK18, cyclin dependent kinase 18, is predicted to enable ATP binding activity and to be involved in protein phosphorylation and regulation of transcription involved in G1/S transition of mitotic cell cycle. It may play a role in signal transduction cascades in terminally differentiated cells. [provided by Alliance of Genome Resources, Apr 2022]
Target class
Kinase
Family
CMGC
Accession number
NM_212502.1. The recombinant protein contains one residue which is in conflict with NM_212502.1. This is T166M and is reported in GenBank BC011526.1.
Target Name
CDK18/cyclin Y, active
Target Alias
Cyclin Dependent Kinase 18; PCTAIRE3; PCTK3; Serine/Threonine-Protein Kinase PCTAIRE-3; PCTAIRE Protein Kinase 3
Origin
Human
Theori. MW
81 kDa (CDK18), 67 kDa (cyclin Y)
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
72% by SDS-PAGE and Coomassie blue staining.
Purification method
glutathione agarose
Sample Buffer
3.85 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Chemical data
Background
CDK18, cyclin dependent kinase 18, is predicted to enable ATP binding activity and to be involved in protein phosphorylation and regulation of transcription involved in G1/S transition of mitotic cell cycle. It may play a role in signal transduction cascades in terminally differentiated cells. [provided by Alliance of Genome Resources, Apr 2022]
Compound name
Kinase
Catalog number
15-031
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
15-031
Datasheet