HRI, active
N-terminal GST-tagged recombinant, human HRI amino acids 140-end.
No items found.
Biological information
Background
HRI (Heme-Regulated eIF2α Kinase) is a serine/threonine protein kinase that is involved in the regulation of protein synthesis in response to changes in heme levels in the cell. HRI is activated in response to decreased heme levels, which can occur during conditions such as iron deficiency, and it phosphorylates the alpha subunit of eukaryotic initiation factor 2 (eIF2α), resulting in a decrease in global protein synthesis and increased translation of mRNAs that contain upstream open reading frames (uORFs) in their 5' untranslated regions. Research has suggested that HRI may also play a role in the response to cellular stress, such as oxidative stress or viral infection. Additionally, HRI has been suggested as a potential therapeutic target in diseases such as sickle cell anemia, where the regulation of hemoglobin synthesis is disrupted, and in viral infections, where the kinase may be involved in the host response to infection.
Target class
Kinase
Family
Ser/Thr Kinase
Accession number
NM_014413.2
Target Name
HRI, active
Target Alias
Origin
Human
Theori. MW
83 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 73% by SDS-PAGE and Coomassie blue staining.
Purification method
Glutathione agarose affinity chromatography; Gel filtration
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
HRI (Heme-Regulated eIF2α Kinase) is a serine/threonine protein kinase that is involved in the regulation of protein synthesis in response to changes in heme levels in the cell. HRI is activated in response to decreased heme levels, which can occur during conditions such as iron deficiency, and it phosphorylates the alpha subunit of eukaryotic initiation factor 2 (eIF2α), resulting in a decrease in global protein synthesis and increased translation of mRNAs that contain upstream open reading frames (uORFs) in their 5' untranslated regions. Research has suggested that HRI may also play a role in the response to cellular stress, such as oxidative stress or viral infection. Additionally, HRI has been suggested as a potential therapeutic target in diseases such as sickle cell anemia, where the regulation of hemoglobin synthesis is disrupted, and in viral infections, where the kinase may be involved in the host response to infection.
Compound name
Kinase
Catalog number
16-013
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
16-013
Datasheet