Cdc7/cyclin B1, active
N-terminal GST-tagged recombinant, human Cdc7, full length and N-terminal GST-tagged cyclin B1, full length.
No items found.
Biological information
Background
CDC7 is predominantly localized in the nucleus and is a cell division cycle protein with kinase activity. Although expression levels of the protein appear to be constant throughout the cell cycle, the protein kinase activity appears to increase during S phase. It has been suggested that the protein is essential for initiation of DNA replication and that it plays a role in regulating cell cycle progression.
GenBank NM_003503.2 / GenBank NM_031966.3
Target class
Kinase
Family
CMGC
Accession number
NM_003503.2; NM_031966.3
Target Name
Cdc7/cyclin B1, active
Target Alias
Receptor Interacting Serine/Threonine Kinase 1Receptor (TNFRSF)-Interacting Serine-Threonine KinaseReceptor-Interacting Serine/Threonine-Protein Kinase 1Receptor-Interacting Protein Kinase 1Serine/Threonine-Protein Kinase RI
Origin
Human
Theori. MW
92 kDa (Cdc7), 75 kDa (cyclin B1)
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 65% (combined) by SDS-PAGE and Coomassie blue staining.
Purification method
Glutathione agarose affinity chromatography
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
CDC7 is predominantly localized in the nucleus and is a cell division cycle protein with kinase activity. Although expression levels of the protein appear to be constant throughout the cell cycle, the protein kinase activity appears to increase during S phase. It has been suggested that the protein is essential for initiation of DNA replication and that it plays a role in regulating cell cycle progression.
GenBank NM_003503.2 / GenBank NM_031966.3
Compound name
Kinase
Catalog number
16-025
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
16-025
Datasheet