LIMK2, active
10 µg of active LIMK2. N-terminal 6His-tagged, recombinant, human LIMK2 amino acids 2-end.
Biological information
Background
LIMK2, LIM domain kinase 2, is a serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. This kinase has also been implicated in brain development. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
TKL
Accession number
NM_005569.4
Target Name
LIMK2, active
Target Alias
LIM Domain Kinase 2; LIMK-2
Origin
Human
Theori. MW
76 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
82% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
0.65 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Chemical data
Background
LIMK2, LIM domain kinase 2, is a serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. This kinase has also been implicated in brain development. [provided by RefSeq, Jul 2008]
Compound name
Kinase
Catalog number
16-032
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
16-032
Datasheet