CK1α, active
10 µg of active CK1α. N-terminal FLAG-tagged, N-terminal 6His-tagged, recombinant, human CK1 alpha, full length.
Biological information
Background
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. CKIa enables protein serine/threonine kinase activity. CK1a is also involved in several processes, including negative regulation of canonical Wnt signaling pathway; peptidyl-serine phosphorylation; and positive regulation of proteasomal ubiquitin-dependent protein catabolic process. Located in centrosome; cytosol; and nuclear speck. Part of beta-catenin destruction complex. Colocalizes with keratin filament and mRNA cleavage and polyadenylation specificity factor complex. Biomarker of Alzheimer's disease and inclusion body myositis. [provided by Alliance of Genome Resources, Apr 2022]
Target class
Kinase
Family
Accession number
L37042.1
Target Name
CK1α, active
Target Alias
CSNK1A1Casein Kinase 1 Alpha 1CKIalphaHLCDGP1PRO2975HEL-S-77p
Origin
Human
Theori. MW
42 kDa
Affinity tag
FLAG, 6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
67% by SDS-PAGE and Coomassie blue staining.
Purification method
Purified using immuno-affinity chromatography and immobilized metal affinity
chromatography.
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Chemical data
Background
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. CKIa enables protein serine/threonine kinase activity. CK1a is also involved in several processes, including negative regulation of canonical Wnt signaling pathway; peptidyl-serine phosphorylation; and positive regulation of proteasomal ubiquitin-dependent protein catabolic process. Located in centrosome; cytosol; and nuclear speck. Part of beta-catenin destruction complex. Colocalizes with keratin filament and mRNA cleavage and polyadenylation specificity factor complex. Biomarker of Alzheimer's disease and inclusion body myositis. [provided by Alliance of Genome Resources, Apr 2022]
Compound name
Kinase
Catalog number
16-050
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
16-050
Datasheet