GAK, active
10 µg of active GAK. N-terminal FLAG-tagged and N-terminal 6His-tagged, recombinant, human GAK amino acids 12-347.
Biological information
Background
GAK (Cyclin-G-associated kinase) is a serine/threonine protein kinase that is involved in the regulation of several cellular processes, including cell cycle progression, endocytosis, and Golgi complex organization. GAK has been shown to phosphorylate several key proteins involved in the regulation of the cell cycle, including cyclin-dependent kinase 1 (CDK1) and its regulatory subunit cyclin B1. Research has suggested that GAK may be involved in the development and progression of several diseases, including cancer and neurodegenerative disorders such as Parkinson's disease. In cancer, GAK has been shown to be upregulated in several types of cancer, and may play a role in the regulation of cancer cell growth and survival. In Parkinson's disease, GAK has been implicated in the regulation of the clearance of α-synuclein, a protein that forms abnormal aggregates in the brain of Parkinson's disease patients.
Target class
Kinase
Family
OTHER-NAK
Accession number
NM_005255.4
Target Name
GAK, active
Target Alias
Cyclin G Associated Kinase; DNAJC26; Cyclin-G-Associated Kinase; Auxilin-2; DNAJ26
Origin
Human
Theori. MW
40.2 kDa
Affinity tag
FLAG, 6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
90% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Chemical data
Background
GAK (Cyclin-G-associated kinase) is a serine/threonine protein kinase that is involved in the regulation of several cellular processes, including cell cycle progression, endocytosis, and Golgi complex organization. GAK has been shown to phosphorylate several key proteins involved in the regulation of the cell cycle, including cyclin-dependent kinase 1 (CDK1) and its regulatory subunit cyclin B1. Research has suggested that GAK may be involved in the development and progression of several diseases, including cancer and neurodegenerative disorders such as Parkinson's disease. In cancer, GAK has been shown to be upregulated in several types of cancer, and may play a role in the regulation of cancer cell growth and survival. In Parkinson's disease, GAK has been implicated in the regulation of the clearance of α-synuclein, a protein that forms abnormal aggregates in the brain of Parkinson's disease patients.
Compound name
Kinase
Catalog number
16-055
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
16-055
Datasheet