GRK4, active
10 µg of active GRK4. C-terminal 6His-tagged, recombinant, human GRK4 full length.
Biological information
Background
GRK4 (G protein-coupled receptor kinase 4) is a serine/threonine protein kinase that belongs to the GRK family of kinases. It is primarily expressed in the kidney, where it plays a role in the regulation of blood pressure and sodium homeostasis. GRK4 has been implicated in the development of hypertension and renal disease, as its upregulation or over activation can lead to an increase in blood pressure and sodium reabsorption. Research has also suggested that GRK4 may play a role in the pathogenesis of several other diseases, including heart failure and obesity. In heart failure, GRK4 has been shown to be upregulated, and may contribute to the development of cardiac dysfunction. In obesity, GRK4 has been implicated in the regulation of adipocyte differentiation and metabolism, and may play a role in the development of insulin resistance.
Target class
Kinase
Family
AGC
Accession number
BC117320.1
Target Name
GRK4, active
Target Alias
G Protein-Coupled Receptor Kinase 4; GPRK4; GPRK2L; GRK4a
Origin
Human
Theori. MW
67.7 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
64% by SDS-PAGE and Coomassie blue staining
Purification method
Immobilised metal affinity chromatography
Sample Buffer
enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Chemical data
Background
GRK4 (G protein-coupled receptor kinase 4) is a serine/threonine protein kinase that belongs to the GRK family of kinases. It is primarily expressed in the kidney, where it plays a role in the regulation of blood pressure and sodium homeostasis. GRK4 has been implicated in the development of hypertension and renal disease, as its upregulation or over activation can lead to an increase in blood pressure and sodium reabsorption. Research has also suggested that GRK4 may play a role in the pathogenesis of several other diseases, including heart failure and obesity. In heart failure, GRK4 has been shown to be upregulated, and may contribute to the development of cardiac dysfunction. In obesity, GRK4 has been implicated in the regulation of adipocyte differentiation and metabolism, and may play a role in the development of insulin resistance.
Compound name
Kinase
Catalog number
16-057
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
16-057
Datasheet