UBE1 active
N-terminal 6His-tagged, recombinant human UBE1 full length, expressed by baculovirus in Sf21 insect cells.
Biological information
Background
UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation, and is responsible for the primary ubiquitin-protein isopeptide bond formation. UBE1 activates ubiquitin by the ATP dependent adenylation of its C-terminal glycine carboxyl group. This ubiquitin residue is then linked to the sulphydryl side chain moiety of a cysteine residue within UBE1 by forming a high energy thiol ester bond, liberating free AMP. There are two active sites within the UBE1 molecule allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade. Expected Purity 94% by SDS-PAGE and Coomassie blue staining.
Target class
Ubiquitin
Family
E1
Accession number
GenBank NM_003334
Target Name
UBE1 active
Target Alias
UBE1, UBA1, CFAP124, UBE1X, POC20, A1S9T, GXP1, AMCX1, SMAX2, UBA1A, VEXAS, A1S9, A1ST
Origin
Human
Theori. MW
122 kDa
Affinity tag
N-Terminal 6His
Product specifications
Expression system
expressed in insect cells
Purity
94%
Purification method
Immobilised metal affinity chromatography
Sample Buffer
2.48 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation, and is responsible for the primary ubiquitin-protein isopeptide bond formation. UBE1 activates ubiquitin by the ATP dependent adenylation of its C-terminal glycine carboxyl group. This ubiquitin residue is then linked to the sulphydryl side chain moiety of a cysteine residue within UBE1 by forming a high energy thiol ester bond, liberating free AMP. There are two active sites within the UBE1 molecule allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade. Expected Purity 94% by SDS-PAGE and Coomassie blue staining.
Compound name
Ubiquitin
Catalog number
23-021
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-021
Datasheet