SCFSkp2/Cks1 complex, active
Complex of N-terminal 6His-tagged, recombinant human Skp2 full length, N-terminal GSTtagged, recombinant human Skp1 full length, N-terminal 6His-tagged, recombinant human Cul1 full length and untagged recombinant human Rbx1 full length, coexpressed by baculovirus in Sf21 insect cells.
Biological information
Background
The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1 and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCFSkp2 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. It specifically recognizes phosphorylated p27 and is involved in regulation of G1/S transition.
Target class
Ubiquitin
Family
E3
Accession number
GenBank NM_005983 Skp2
Target Name
SCFSkp2/Cks1 complex, active
Target Alias
FBL1, FBXL1, FLB1, p45, Ckshs1
Origin
Human
Theori. MW
11 kDa (Cks1), 52 kDa (Skp2), 46 kDa (Skp1), 93 (Cul1) and 12 kDa (Rbx1)
Affinity tag
N-terminal 6His-tag Skp2 / N-Terminal GST-tag Skp1 / N-Terminal 6His-tag Cul1 / untagged Rbx1
Product specifications
Expression system
expressed in Insect (SCFSkp2)/E.coli (Cks1)
Purity
92%
Purification method
Immobilized metal affinity chromatography
Sample Buffer
1.11 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1 and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCFSkp2 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. It specifically recognizes phosphorylated p27 and is involved in regulation of G1/S transition.
Compound name
Ubiquitin
Catalog number
23-023
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-023
Datasheet