p27 complex, activated
Complex of N-terminal c-Myc-tagged, recombinant human p27 full length, 6His-tagged, recombinant human Cyclin E1 full length and untagged, recombinant human CDK2 full length, coexpressed by baculovirus in Sf21 insect cells.
Biological information
Background
p27 is an important regulator of cell cycle progression, involved in G1 arrest. It is a potent inhibitor of cyclin E1- and cyclin A-CDK2 complexes and a positive regulator of cyclin D-dependent kinases such as CDK4. The degradation of this protein, which is triggered by its CDK-dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Decreased levels of p27 are found in various epithelial tumours originating from lung, breast, colon, ovary, oesophagus, thyroid and prostate. Defects in the gene encoding p27 (CDKN1B) are the cause of multiple endocrine neoplasia type 4 (MEN4) an inherited cancer of the thyroid.
Target class
Ubiquitin
Family
Substrate
Accession number
GenBank BC001971 p27
Target Name
p27 complex, activated
Target Alias
CDKN4, MEN1B, P27, MEN4, KIP1, CDKN1B, P27KIP1, P27Kip1
Origin
Human
Theori. MW
23 kDa (p27), 34 kDa (CDK2) and 48 kDa (Cyclin E1)
Affinity tag
N-terminal c-Myc-tag p27 / 6His-tag Cyclin E1 / untagged CDK2
Product specifications
Expression system
expressed in insect cells
Purity
95%
Purification method
Immobilised metal affinity chromatography
Sample Buffer
0.44 mg/ml of enzyme in 25 mM MOPS pH 7.5, 5 mM MgCl2, 0.01% Tween 20, 1 mM ATP. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
p27 is an important regulator of cell cycle progression, involved in G1 arrest. It is a potent inhibitor of cyclin E1- and cyclin A-CDK2 complexes and a positive regulator of cyclin D-dependent kinases such as CDK4. The degradation of this protein, which is triggered by its CDK-dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Decreased levels of p27 are found in various epithelial tumours originating from lung, breast, colon, ovary, oesophagus, thyroid and prostate. Defects in the gene encoding p27 (CDKN1B) are the cause of multiple endocrine neoplasia type 4 (MEN4) an inherited cancer of the thyroid.
Compound name
Ubiquitin
Catalog number
23-024
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-024
Datasheet